In order to simulate the nanoparticle deposition in the human lung, this study developed an electrostatic Wet Nanoparticle Sampler (WNS) to collect atmospheric and laboratory produced nanoparticles. Electric filed was then apply to deposit nanoparticles suspended in the liquid on the pre-cultured cell by a newly developed Cell Electrostatic Precipitator (Cell-ESP) based on the electrophoretic theory. Finally, the cell index of the exposed cell lines was measured by a real-time cell growth analyzer to determine the nanotoxicity (in cooperation with Prof. Chueh of Chung Hsing University). The combination of the WNS and Cell-ESP is the nanoparticle cell toxicity testing platform developed in this study. As the results of this study, we demonstrated that the samples of environmental nanoparticle could be collected accurately and good chemical mass closure (CMC) could be obtained. The research results were published in four journal papers in world renowned Atmospheric Environment and Aerosol Science and Technology. This year, we continued to conduct the samplings at the locations where city residents, students, drivers, tourists as well as workers were likely to expose to nanoparticles and at a workplace where nano-BC was used. In addition, the characteristatics of nanoparticles were studied at the LuLin background site and inside and at the outlet of SyueShan tunnel. It is expected that the long rang transport of air pollutants from biomass burning in Southeast Asia, and nucleation and formation of SOA (secondary organic aerosol) can be better understood, and the exposoure levels of the residents to engine nanoparticles can be quantified. This study also reviewed the latest literature on the measurement and characterization methods for environmental nanomaterials in the air and water. The summary report will be submitted to the environmental nanotechnology knowledge plateform of Taiwan EPA for publication and public use.

nanoparticle sampling and analysis;mass closure of nanoparticles;EHS of nanomaterials;nanotoxicity;PAHs denuder